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Luciferase reporter assays A bicistronic vector pcDNA3 RLuc POLIRES FLuc (empty reporter) (Addgene, 45642) and pcDNA3 RLuc POLIRES FLuc containing human PRKAA1 5UTR upstream of FLuc (5UTR reporter) were transfected into shCtrl and shGDH1 or shYBX1 Huh7 cells using Lipofectamine 2000 (Thermo Fisher)

It binds with high affinity to HGF inducing dimerization and Met phosphorylation in the presence of subthreshold levels of HGF [Figure 2] [74]

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Specifically, wild-type p53 expression can decrease the expression of PDL1 by increasing the expression of miR34a and promoting the expression of the natural killer (NK) cell-activating ligands UL16-binding protein 1 (ULBP1) and ULBP2