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156 In vivo studies have also shown that aspirin reduces angiogenesis, decreasing VEGF expression and vascular density in A549 xenografts, suggesting its role in tumor growth suppression and angiogenesis inhibition in NSCLC

They were subsequently allowed to rest at room temperature for a total of 20 minutes

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The cells were grown at 27 C with shaking at 170 rpm for 72 h, at the end of which the cells were harvested by centrifugation and stored at 80 C