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The remaining homogenate was centrifuged at 40,000 g for 60 min, and the supernatant was used for the estimation of superoxide dismutase (SOD) and catalase (CAT)

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Heat stress directly damages spermatogenic cells and triggers pathological changes, including oxidative stress, disruption of the bloodtestis barrier, and a proteostasis imbalance, further affecting spermatogenesis (Qin et al., 2026)