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Reported reactions included nausea, vomiting, chills, low blood pressure, breathing difficulty and hospitalization, with excessive endotoxin exposure identified as a concern

Isoform products were separated on 10% polyacrylamide gels and stained with SYBR gold (Invitrogen) and quantified with Image Lab (Bio-Rad)

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Monitoring should be straightforward: subjective pain and function reporting at four weeks, basic labs if comorbidities warrant