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tirzepatide averages ~20% Administration: Phentermine is an oral daily pill
Briefly, treated cells were harvested and lysed in RIPA lysis buffer (Fude, Wuhan, China)
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Embodiment 2 [0079] Embodiment 2 Purification of compound I crude product [0080] Weigh 500 mg of the crude compound I prepared in Example 1, add 15 mL of distilled water to dissolve, use Water1525 system for semi-preparative purification, the wavelength is 214 nm, and the chromatographic column is 20 250 mm reverse phase C 18 Column, column temperature is 37 , mobile phase is the water (A phase) that contains 0.1% TFA and the acetonitrile (B phase) that contains 0.1% TFA, flow velocity is 8ml / min, gradient: B%: 20%-60%, After 30 min, the target components were collected, and the collected solution was concentrated under reduced pressure
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