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Following lyophilized proteins were used: human serum albumin (HSA) from Sigma/Merck (Darmstadt, Germany), alpha-2 macroglobulin (2M) from human plasma (slow form and fast form) and ceruloplasmin (CP) from human plasma were from Athens Research and Technology (Athens, USA), 2M was purchased also from Sigma/Merck (Darmstadt, Germany)

That is why the date rule exists alongside the visual check rather than instead of it

This framing is essential for more effective, less stigmatizing clinical communication and for long-term management strategies

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