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Conclusion Whilst severe B12 deficiency has a clear clinical picture with characteristic haematological and/or neurological features, establishing mild or borderline deficiency can be problematic as serum levels are maintained at the expense of tissue concentrations, and therefore serum levels of B12 may be in the low normal or borderline range despite a deficiency

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Traumatic brain injuries in a well-defined population: epidemiological aspects and severity

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The plates were incubated for 1 h at room temperature, and after the end of this period, the respective anti-material antibodies conjugated to the biotin were added to the plates and incubated for 1 h at room temperature