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LC-MS measurement of HepG2 proteome Peptides were measured and online-separated using an UltiMate 3000 nano HPLC system ( Dionex ) coupled to a Bruker timsTOF Pro mass spectrometer via a CaptiveSpray nano-electrospray ion source and Sonation column oven
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In laboratory studies, it is sometimes used alongside other metabolic or mitochondrial research agents, depending on experimental design
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