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For example, to achieve specific delivery of Cas9 mRNA and sgRNA to tissues, multi-component LNPs were engineered using ionizable phospholipids that can disrupt cellular membranes [142]

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This extension not only increased the molecular weight (from 7.6 kDa to 9.1 kDa), but also reduced the affinity for IGF binding proteins (IGFBPs) through steric hindrance effect

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