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Thermo Fisher Scientific) containing 2% (v/v) Matrigel (Corning) and seeded in a non-cell-adhesive round-bottom 96-well plate (Primesurface 96U plate
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To further determine whether GLP-1R and PKA signalling were involved in the regulation of metformin functions in HUVECs, the protein levels of GLP-1R and phosphorylated PKA (p-PKA) were examined
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