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Islets were purified using an Optiprep gradient (Nycomed, Oslo, Norway) as previously described 81 and cultured for 18 h in a 37 C in a 5% CO 2 incubator in Roswell Park Memorial Institute (RPMI) medium supplemented with 10% heat-inactivated fetal bovine serum before use in experiments
Safety, Side Effects And Who Should Avoid It Safety, side effects and who should avoid it include: Common Side Effects Nausea, stomach cramps, or diarrhoea Fishy body odour due to the metabolite trimethylamine Occasional headache or restlessness Less Common But Important Cautions Seizures: Rare reports link carnitine to increased seizure activity in people with seizure disorders
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