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Peer review Peer review information Nature Reviews Cardiology thanks Luigi Fontana and the other, anonymous, reviewers for their contribution to the peer review of this work
Real-time quantitative polymerase chain reaction Total RNA was extracted from cells using the Trizol reagent (Takara Bio Inc, Japan) according to the manufacturers instructions
Obesity significantly elevates the risk of conditions such as type 2 diabetes, hypertension and cardiovascular disorders, creating stronger demand for treatments capable of producing sustained weight loss and improving overall metabolic health
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However, approval usually requires prior authorization, detailed lab results, and formulary confirmation
Further, the external utility of our findings may vary, especially outside the VA in settings where data toolsets do not already exist, clinical pharmacists with prescriptive authority are less available, or other barriers to use exist, such as higher copayments and more stringent prior authorization requirements
