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Brain imaging studies suggest altered serotonin synthesis and signaling in regions involved in the corticostriatothalamocortical (CSTC) loops, circuits that are central to obsessions and compulsions
After completion of the isoelectric focusing, the strip was equilibrated in the equilibration solution as described by Barraclough [37] and placed on a polyacrylamide gel for SDS-PAGE with a Protean II unit (Bio-Rad, USA) at a constant voltage (300 V)
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