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Realtime quantitative PCR Total RNA from liver samples or cultured cells was extracted using mirVana miRNA isolation kit (Ambion) and DNase treated with Turbo DNAfree DNase Treatment kit (Ambion) as per manufacturer's protocol

The procedure is more painful, carries higher risk of nerve or vascular puncture, and often produces post-injection soreness lasting 1224 hours

Standard oral forms break down before reaching the bloodstream, making them largely ineffective

[179], daily CTP supplementation in healthy adults improved lipid metabolism and vascular indices associated with atherosclerosis risk

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