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A volume of 4-NPG (final concentration of 1 mM) was added to the reaction and decrease in absorbance at 305 nm was recorded on an Envision multilabel plate reader (Perkin Elmer)
These are agonists, meaning that they act on the receptors, but they are not the exact molecule, which have been utilized now for many years for weight loss and diabetes control
continuous administration leads to receptor downregulation and, paradoxically, suppresses testosterone
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