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High levels of these biomarkers suggest B12 deficiency, even with normal serum B12
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This is why peptides are almost always shipped in vials that protect from light and why we recommend keeping them in a dark place (like their original box) inside the refrigerator
For the reduction of disulfide bonds, DTT (15 l, 31 mg ml 1 in ddH 2 O) was added to the samples and incubated at 37 C for 45 min with constant shaking (850 rpm, Thermomixer
You can [also] contaminate the vial and inject it into your stomach and get an infection
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