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To transform the library plasmid into the desired selection strain, selection strains (S2060 (pBAD-protease) derivatives) were grown overnight in 5 ml of DRM supplemented with 50 g ml 1 spectinomycin and 12 g ml 1 tetracycline
2.5 mg dose in a 20 mg vial: Add 2 mL BAC water = 25 units (0.25 mL draw)
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