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Fifty microliters of stop solution was added to each well and the optical density at 450 nm determined using a microtiter plate reader (Liuyi, Beijing, China) within 15 min
The interaction of these neurons, specifically the lateral parabrachial nucleus pathway, needs to be further elucidated in future studies
Valenti L, Fracanzani AL, Dongiovanni P, et al
But the importance of the latter in stimulating appetite cannot be ignored altogether
Close the lid tightly after use to prevent drying
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