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Tissue samples: Homogenization lysis was performed using a suitable buffer (PBS, saline, etc.), where a ratio of 1 to 9 (0.1 g: 0.9 mL) of tissue to saline was sufficient

Those most at risk include vegans and vegetarians, people with digestive conditions such as Crohn's disease, coeliac disease or IBS, those taking long-term metformin or proton pump inhibitors, older adults whose ability to absorb B12 through the gut declines naturally with age, those with pernicious anaemia, and anyone with a history of chronic alcohol use

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