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Full MS scans were acquired in positive and negative ionization mode with the following settings: spray voltage 3500 V/ 2500 V, sheath gas 40 arb units, aux gas 10 arb units, sweep gas 1 arb unit, ion transfer tube 300C, vaporizer temperature 370C, EASY-IC run-start, default charge state 1, resolution at m/z 200 120.000, normalized AGC target 100%, maximum injection time 100 ms, RF lens 35%
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Administration of B12 Injection : The injection is typically administered in the upper arm or thigh