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200 pl sample was transferred to a well in a 96-well microtiter plate

What Happens When Recycling Slows Down In patients, the enzyme GR, which is responsible for recycling spent/oxidised glutathione back to fully loaded, slows down, which leads to the accumulation of oxidised glutathione (GSSG) and the depletion and inability to produce GSH

Glutathione expels toxins in a form that can be excreted through the bile and stool, and it recycles other antioxidants including vitamins C and E

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As NAD+ declines with age, sirtuin activity is reduced, compromising all downstream functions