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For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)

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Gradient Recrystallisation Precision Refinement: High purity crystalline is obtained after removal of trace isomer impurities