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albicans (SC5314 and 101) overnight cultures were seeded in 200 L RPMI-1640 for Seahorse (Agilent Technologies, Santa Clara, CA), supplemented with 2 mM l-Glutamine, 1 mM sodium pyruvate and 11 mM d-Glucose with or without 10 mg/mL albumin (Sanquin Plasma Products B.V.) as 10 4 yeast cells/well in a Seahorse 96-well cell culture plate
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When introduced into the bloodstream, it has a half-life of only a few minutes
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