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We extracted the following 30 factors: age, sex, body mass index (BMI), race (Asian, American Indian or Alaska Native, Black or African American, Native Hawaiian or Other Pacific Islander, White, Other Race, unknown), diagnoses (heart failure, ischemic heart diseases, old myocardial infarction, atrial fibrillation and flutter, hypertension, dyslipidemia, chronic kidney disease, cerebral infarction), and medication (be-ta blockers, diuretics, mineralocorticoid receptor antagonists [MRA], angiotensin converting enzyme [ACE] inhibitors, angiotensin II receptor blockers [ARB], sacubitril, statins, metformin, sulfonylureas, sodium-glucose co-transporter 2 [SGLT2] inhibitors, insulin, laboratory (systolic blood pressure, diastolic blood pressure, glomerular filtration rate [GFR], hemoglobin, albumin, LDL cholesterol, hemoglobin A1c [HbA1c])

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Quality control of recovered PCR product was performed on the Agilent 2100 Bioanalyzer, and cDNA yield was measured by Qubit DNA high-sensitivity assay (Invitrogen, Carlsbad, CA, #Q32854)

While the program is not required, it is highly recommended

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