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Following each round of selection, genes encoding the active peptides were recovered from sorted cells by PCR and subcloned into a lentiviral vector to generate an enriched library used for the next round of selection

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And it means improved hormone regulation, since growth hormone, which is critical for body composition, is released primarily during deep sleep

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Electrophysiology Neurons were constantly perfused at 3 ml min 1 with heated (3032 C), gassed (95% O 2 , 5% CO 2 ) artificial cerebrospinal fluid (pH 7.4, 300305 mOsm) containing (in mM): 119 NaCl, 2.5 KCl, 1.3 MgCl 2 , 2.5 CaCl 2 , 26 NaHCO 3 , 1.3 NaH 2 PO 4 , 20 D-glucose and 0.5 sodium ascorbate