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Radioligand and fluorescent binding assays, competition binding, recombinant receptor-expressing cell lines, functional readouts through a defined coupling pathway, and antagonist controls demonstrating that an effect is receptor-mediated

Safety and Monitoring : Given the potential risks, especially related to tumor growth due to increased angiogenesis, it is crucial to monitor health metrics and use the minimum effective dose

Published work reports activity at concentrations orders of magnitude below those required for brain-derived neurotrophic factor in comparable assays