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This rigorous analytical validation verifies the exact tripeptide sequence composition (Ala-His-Lys), precise molecular weight, and correct spatial conformation while ensuring the total absence of residual synthesis reagents, truncated fragments, heavy metals, or raw contaminants

Administration routes tested included both intravenous injection and oral formulations delivered as capsules or tablets

K-means clustering Five clusters were used for k-means clustering, per the results of the sum of squared estimate of errors curve, on the ASV relative abundance data with clustering by animal (n = 25)

Food intake and body weight were measured twice weekly

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