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In addition, estrous cycles were monitored by daily vaginal cytology during the last 12 days of intervention with the different compounds, using well-validated protocols for histological identification of the prototypical cellularity at each phase of the cycle

While possible, maintaining results after stopping GLP-1 therapy is challenging and requires intensive lifestyle modifications including 200-300 minutes of weekly exercise, consistent dietary changes, regular self-monitoring, and often ongoing behavioral support

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Individual variation in this timeline depends on factors including body composition, kidney and liver function, and metabolic rate

3 , e12541 (2015)