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Across that run-in, participants achieved a mean weight reduction of 10.6%

Some frequent damaging consequences of high temperature include reduced germination and biomass, increased tillering, chlorosis, necrosis, early senescence of floral buds, premature mortality, and fruit senescence

Stable EndoC-H1 shRNA cell sublines were generated by infection of the parental EndoC-H1 line 40 with lentiviral particles expressing shRNA duplexes previously cloned into pLKO.3G (Addgene #14748)

Metabolic analysis was performed on a Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher) coupled to a Vanquish UHPLC System (Thermo Fisher)

Most people find themselves hungry again quickly after eating, which makes the protocol difficult to sustain even over a single day