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In particular, ensuring adequate CNS exposure, maintaining appropriate isoform selectivity margins, and establishing a tolerable safety profile are essential requisites for its development as a drug (Al-Nema et al., 2021)
10.1016/S0891-5849(02)01009-2
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At an OD 600 of 0.60.7, expression was induced with 1 mM IPTG and the culture was continued up to an OD 600 of 3.0 where the cells were harvested at the end of the exponential growth phase by centrifugation (10 min, 3,220 g , 4C)
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