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Samples were fixed in 3.7% paraformaldehyde (PFA) at 4C for 24 h and then decalcified in 500 mM ethylenediaminetetraacetic acid (EDTA) (pH 7.4, VWR, Pennsylvania, USA) 66
Studies in dogs have shown that the IL-6 and TNF- levels tend to increase with age, suggesting that inflammaging may play a major role in the aging process of dogs [110, 111]
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