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doi: 10.3389/fphar.2021.637415 44 ChangWCJan WuYJChungWHLeeYSChinSWChenTJet al

For the next measurement pellets are immersed in a buffer not containing ADP and the TL is biased away from ADP pickup and towards ATP transfer into the artificial cytosol at pH = (8.9 0.2) After 10 min the mitochondrial ATP is again measured giving result k, and the factor TL IN is the fractional decrease: l = [(g k) / g]

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