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This prevents introducing contaminants during the mixing procedure
Flow cytometry quantification of cell viability of HC lymphocytes, monocytes, and neutrophils cultured with 20% HC or SLE serum for 16 hours and the proportion of apoptotic (Annexin V + 7AAD-), necrotic (Annexin V + 7AAD + ) and live (Annexin V- 7AAD-) cells in each subset was analyzed (n = 6)
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